• Gary Brewer
  • Gary Brewer
  • Professor
  • Department: Department of Biochemistry & Molecular Biology
  • Program(s): Biochemistry Graduate Program, Cell and Developmental Biology Graduate Program, Cellular and Molecular Pharmacology Graduate Program, Microbiology and Molecular Genetics Graduate Program
  • Phone: 1.7322353473
  • Robert Wood Johnson Medical School
  • 675 Hoes Lane
  • Piscataway, NJ 08854-5635
  • Key Words: Post-transcriptional control of gene expression in cancer, immune responses, and congestive heart failure

Rapid mRNA decay results from cis-acting elements present in labile mRNAs. For example, many mRNAs encoding oncoproteins, cytokines, and G protein-coupled receptors are labile due to the presence of A+U-rich elements (AREs) in their 3'-UTRs. We developed and have utilized a cell-free mRNA decay system to biochemically dissect the turnover machinery. Using this system we identified, purified and molecularly cloned an RNA-binding protein, AUF1, which effects ARE-directed mRNA decay via its high-affinity binding to AREs. We have a number of projects ongoing in my lab. (1) We are utilizing the cell-free mRNA decay system to dissect how AUF1-ARE interactions target mRNAs for rapid decay. (2) We are utilizing a cell line deficient in AUF1activity to ectopically express AUF1 and examine the effects on ARE-directed mRNA decay. (3) AUF1 levels are elevated in hearts of patients with congestive heart failure compared to normal hearts. By contrast, the levels of ß1-adrenergic receptor (ß1AR) mRNA, which contains an ARE, and protein are lower in failing hearts. Because the ß1AR protein is essential for cardiac output, we believe that elevated levels of AUF1 in the failing heart is detrimental to cardiac function. We are currently developing a transgenic model of cardiac AUF1 overexpression to further address this relationship. (4) We are examining how MAP kinase pathways affect AUF1 activity to stabilize interleukin-1ß and tumor necrosis factor-alpha mRNAs in monocytes that become adherent at sites of inflammation and tissue injury. (5) Finally, we are utilizing physical methods, such as measuring changes in fluorescence anisotropy, to dissect the molecular details of the binding of AUF1 to its cognate RNA sequences.